KMID : 1137820060270050218
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ÀÇ°øÇÐȸÁö 2006 Volume.27 No. 5 p.218 ~ p.223
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Surface Immobilization of $(1{\to}3)(1{\to}6)-{\beta}-glucan$ onto Biodegradable Polymer for Tissue Regeneration
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Lee Sang-Kil
Lee J.B. Yu S.M. Park Jong-Chul Choi J.B. Kim J.-K.
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Abstract
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We examined the effects of ${\beta}$-glucan-reinforced PLGA film and scaffold on HDFs (human dermal fibroblast) attachment and proliferation. The PLGA films were prepared by simple solvent-casting method. The prepared films were grafted with $(1{\to}3)(1{\to}6)-{\beta}-glucan$ in various ratios after plasma treatment on surface. The surface of the film was characterized by contact angle measurement, scanning electron microscope (SEM), and Fourier-transform infrared spectrophotometer (FT-IR). The amount of $(1{\to}3)(1{\to}6)-{\beta}-glucan$ in the prepared film was indirectly determined by phenol-sulfuric acid method. The HDFs (Human dermal fibroblasts) were used to evaluate the cell attachment and proliferation on PLGA specimens before and after plasma/${\beta}-glucan$ treatment. The result showed that the plasma treated groups exhibited more mont of ${\beta}-glucan$ might be grafted than the non plasma treated groups. Cell attachment was significantly enhanced in the plasma/${\beta}-glucan$ grafted group after 4 hours incubation (p<0.05) due to the improved hydrophilicity and cytoactivity effect of the ${\beta}-glucan$. The cell proliferation of plasma/${\beta}-glucan$ (2mg/ml) grafted group was the highest rate among the groups (p<0.05).
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KEYWORD
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tissue regeneration, PLGA, ${\beta}-glucan$, plasma treatment, cell affinity
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